PCV2人工感染对猪肺泡巨噬细胞干扰素信号通路的调控研究

doi:10.11843/j.issn.0366-6964.2016.03.023

Abstract

Abstract:

To understand the mechanism of PCV2 replication and pathogenesis，change and regulation of interferon in alveolar macrophages of piglets subclinically infected with procine circovirus type 2(PCV2) were studied.Fifteen 30-day-old piglets were selected as experiment animals，which were free of PCV2 and porcine reproductive and respiratory syndrome virus(PRRSV) antibody and antigen.The piglets were divided into three groups：control group(0 dpi)，PCV2 infection group(14 dpi) and PCV2 infection group(28 dpi).After infection，the mRNA transcription of interferon，pattern recognition receptors(PRRs) and pathogen associated molecular proteins(PAMPs) in AMs were detected by real-time PCR.The results showed that the mRNA levels of IFN-β and IFN-γ were significantly higher at 14 dpi and 28 dpi(P＜0.05)；the mRNA expression of RIG-1，MDA-5，TLR4 and TLR9 were significantly increased at 14 dpi and 28 dpi(P＜0.05)；the mRNA level of DAI was significantly elevated at 14 dpi(P＜0.05)，and then decreased at 28 dpi；while there were no significant change in mRNA expression of TLR3，TLR7 and TLR8.Compared with control，the mRNA transcription of MAVS，MyD88，IRF3 and IRF7 were significantly increased at 14 dpi and 28 dpi(P＜0.05).These results demonstrate that the up-regulations of IFN-β and IFN-γ in alveolar macrophages of PCV2 subclinical infection are relative to TLR4/TLR9/MyD88 and RIG-1/MDA-5/DAI/MAVS/IRF3 signaling pathways.

CLC Number:

S852.3

ZHAO Qi-ling，ZHANG Xin-chen，CHEN Meng-meng，SUN Jia-rui，BAO En-dong，ZHANG Shu-xia，Lü Ying-jun. Regulation of Interferon Signaling Pathway in Alveolar Macrophages of Piglets Infected with Procine Circovirus Type 2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 587-594.

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Regulation of Interferon Signaling Pathway in Alveolar Macrophages of Piglets Infected with Procine Circovirus Type 2

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